Thursday, January 30, 2020

Sentimental plot Essay Example for Free

Sentimental plot Essay During the 19th century many writers began to publish works with sentimental plots. Generally the sentimental plot is written to make the reader feel an excess of unnecessary emotions, by overemphasizing every work. In every sentimental plot there will always be the typical sentimental hereon that fits the stereotype of a young orphan, unmarried women with moral obstacles. In this plot there are only two types of women the ones that are considered good and the ones that are bad. In the sentimental plot the good will get rewarded by marrying a decent man and the bad will always die. Even though this plot was very popular among writers many women were not happy with how the women were being portrayed among men writers so they decided to go against this plot. With women writers some would tend to go against the sentimental romance plot and instead write about actual situations that were happening around them. One example of this would be in Kate Chopin’s story â€Å"The Storm† it featured a married women falling into the seductions of a old flame. Do you remember in Assumption, Calixta? He asked in a low voice broken by passion. Oh! She remembered; for in Assumption he had kissed her and kissed and kissed her; until his senses would well nigh fail, and to save her he would resort to a desperate flight. If she was not an immaculate dove in those days, she was still inviolate; a passionate creature whose very defenselessness had made her defense, against which his honor forbade him to prevail. † Pg. 59 Author Kate Chopin was unable to publish her works because of the featured adultery with no follow up punishment, in order for her to successfully publish she would have to follow the sentimental romance plot and kill Calixta in the end to prove that the decisions she made were wrong and had consequences. Mary E. Wilkins in â€Å"The Revolt of Mother† is another example that goes against the grain of the sentimental plot it deals with a mother that confronts her husband and goes against his say so which in the 19th century was something that was frowned upon. Now father, said she you needn’t be scared. I ain’t crazy. There ain’t nothing to be upset over. But we’ve come here to live, an’ we’re goin’ to live here. We’ve got jest as good as right here as new horses mind I wa’nt fit for us to live in any longer, an I made up my mind I wa’n’t going’ to stay there. I’ve done my duty by you for forty year, an’ I’m goin’ to do it now; but I’m going to live here. † Pg. 672 After this story was published many people thought that it was based on a true story but Mary E. Wilkins explained that no New  England women would ever dare confront her husband it was all just a fantasy. Another example of the reversed sentimental plot would be in Charlotte Perkins Gilman story â€Å"The Yellow Wall-paper† It is based on a women who is said to be having nervous breakdowns and is being taken care of by her husband a physician she later comes to relies that she will no longer be trapped or hide her emotions. â€Å"I’ve got out at last. Said I, in spite of you and Jane! And I’ve pulled off most off the paper, so you can’t put me back! † pg 803. What all of these story’s have in common is that they tried to convey across the situations that the women were in and all of them one way or another expressed how the women felt trapped by the men. When it came to men writers they would always include a sentimental hereon in their stories. Henry James published â€Å"Daisy Miller† in 1978 and it dealt with a young unmarried woman somewhat considered an orphan because her parents were never around, who was seen in society as an outcast cause of the way she would conduct herself around men. Miss Daisy Miller was a flirt – a pretty American flirt. He had never, as yet, had any relations with young ladies of this category. He had known, her in Europe† pg. 427 since Daisy Miller was portrayed as an uneducated flirt Henry James followed the sentimental plot by giving Daisy a terrible case of the fever and later died. Later after the story was published Henry James stated that he believed that Daisy was innocent and that he did not kill her to state a point, it just so happened that she caught fever. So in some sort of way this story can sometimes be said that it was not your typical sentimental plot. Another male writer that followed the sentimental hereon was William Dean Howells â€Å"Editha† this was about a young unmarried woman who practically forces her boyfriend to enter the war in order to show off in front of others. â€Å"I shall always love you, and therefore I shall never marry any one else. But the man I marry must love his country first of all, and be able to say to me, I could not love thee, dear, so much, loved I not honor more. In these two story’s It seems to me as if the men writers are trying to put women down by making them seem selfish and not following the norm of obeying the men’s commands or ways of lifestyle that they want them to follow. In all of these stories we can see that there is a big difference in how men and women respond to the sentimental romance plot. The women write about there own personal experiences and the things that they see happen around them and the men write about what they want the people to read for example the good will get a great husband and get married and the bad will get punished and die.

Wednesday, January 22, 2020

A Rebel on His Way to Adulthood in The Catcher in the Rye :: The Catcher in the Rye Essays

Ever since I read ‘The Catcher in the Rye’ – I was 17 then – I have tried to explain myself why this book is so significant. Why is it so hated and adored at the same time? I must confess I hadn’t heard anything about Salinger till I watched ‘Conspiracy Theory’. If you don’t remember I’ll tell you that the movie was about this taxi driver Jerry Fletcher who traced conspiracy in everything and all of sudden one of his theories came to be true. Mel Gibson was incredible playing a man who was funny and serious, brilliant and a bid mad. And this queer person couldn’t feel ‘normal’ if he didn’t buy a copy of ‘The Catcher in the Rye’ every day. And that impressed me so much that after that I bought the book myself. I don’t know why I did it. Generally, I hate to be told what to do, less what to read. But at that time perhaps I needed to feel ‘normal’ too. Now, four years later, I read it again. And I was a bit nervous about it. After all there is this disaster called ‘time’ and we are all infected by it but there is no cure. The symptom is that we change but not only physically. Our points of view, ideals and beliefs also change. That is why I was so anxious about reading the book again but at the same time I was curious to find out how much I have changed. Actually, I didn’t remember most of it. I could recall only my feelings. And that is not a recommended approach towards a book. But I don’t consider myself much of a critic but an observer and an interpreter. After all readers, incl. critics who are also readers, respond to a book the way it has affected their inner self. That’s why I would like to trace the difficult path towards adulthood that passes by various social and individual stops. For me, that is what makes the book so significant. Because we all struggled and will continue to struggle such psychological battles on our way towards ‘know thyself’. I would like to begin with the way some critics approached Salinger’s work. Although each of them expressed different points of view which are too extreme I would like to consider them and try to find the truth somewhere in the middle. Ann Goodman commented that ‘Holden was so completely self-centered that any other characters who wandered through the book, with the exception of his sister, Phoebe, had no authenticity at all’.1 Of course, it’s not so surprising for a person that passes such a

Tuesday, January 14, 2020

Frontline: Camera and Current Affairs Programs

How do the creators of â€Å"Frontline† convey the main issues in the text? The truth in the media is a subjective commodity which is often sensationalized, fabricated and manipulated for a number of reasons. Therefore, realistically the media only represents certain facets of the truth to suit their purpose and context. Rob Sitch’s Frontline is a satirical examination of current affairs programs and explores the media’s selective dissemination of information to construct the desired truth. Though being disturbingly accurate in their sarcastic attack on the deceit and hypocrisy of the media, the producers of Frontline use wit and parody to challenge the reliability of the media and such concepts are explored through a number of film techniques in episodes such as ‘The Siege’ and ‘Add sex and stir’ The commercial nature of the media dictates that the content of the broadcast must rate well and be profitable. The episode ‘The Siege’ exemplifies the commercial nature of current affairs programs and re-emphasizes the media’s potential to regard the truth as a subjective commodity to further gain higher ratings. Frontline seeks little more than authenticity to convince their audience that they are a credible resource. In ‘The Siege’ Rob Sitch blurs the boundary between fiction and reality, through illustrating a story based on a real incident which occurred in 1994 making it harder to discern the truth. Frontline pursues a story about a father who has held his children hostage during a custody battle. This episode exposes the true nature of journalism and the unethical practices that the media undertakes to win the ratings war. Marty ironically says ‘you’ve crossed the line mate’, this pretense of ethics is juxtaposed with ‘you beauty’ when he realizes that it was the Frontline team itself that got through to interviewing the gunman. Marty further sensationalizes and exaggerates the truth by wearing a flak jacket and crouching down to make it appear that he is in a dangerous situation, when in reality he is five kilometers away from the danger zone. Through the use of dialogue ‘How about if I crouch down like this? To make it look like I’m in danger’ Marty illustrates his blatant disregard for the truth thus emphasizing the importance of appearance and ratings over the depiction of the truth within the media. Brooke further epitomizes the media’s motives through the use of incongruity in the dialogue ‘Mrs. Forbes do you have any nine volt batteries? ’ Such dialogue accentuates Brooke’s superficiality as she is not at all concern ed about Mrs. Forbes feelings. The camera close up of Brooke’s exceedingly concerned facial expression when she interviews Mrs. Forbes is juxtaposed with Mrs. Forbes misery and Brooke’s deception when she asks – ‘would you be able to cry again Mrs. Forbes? ’ The on and off camera persona evidently shows that the truth is subverted by the media to construct a desired representation, portraying the truth to be a subjective commodity. The media’s relentless pursuit of higher ratings to ensure commercial success plays a significant role in determining the representation of the truth they present to their audience. The episode ‘Add sex and Stir’ epitomizes the media’s selective dissemination of information to construct a desired truth, thus exemplifying truth to be a subjective commodity. Frontline pursues a story about the unfair dismissal of a sportswoman who is supposedly dropped from the team because she is not gay. Although Brooke acknowledges that the sportswoman was dropped from the team because of poor form, she decides to portray the misleading sex angle of the story in the pursuit for higher ratings to ensure Frontline’s commercial success. Frontline’s clean crisp picture quality, still camera and immaculate sets gives a sense of professional respectability and authority to create a sense of credibility. Rob Sitch employs caricature to emphasize the potential for the media to regard the truth as a subjective commodity to gain more ratings. Sitch satirizes the limited value that the media places on the truth through Brooke’s satirical comment ‘Well there’s a lead story, Stu, lead player dropped due to bad form, out breaking. ’ The truth is further manipulated through filming Brooke’s nods separately and out of context which further amplifies their falsity, emphasizing her lack of integrity. Brooke completely distorts the truth through creating a re-enactment, in which the story is further exaggerated through the steamy atmosphere in the girls change rooms, demonstrating that it is sleaze that sells rather than the truth. The dramatic background music in the re-enactment further satirizes the media’s pursuit for higher ratings, portraying that the media’s ‘essential objective is profit, rather than saving the world’ Chris Masters (ABC correspondent). Through this it is clearly evident that the truth to the media is a subjective commodity that can often be manipulated, fabricated and sensationalized for a number of reasons.

Monday, January 6, 2020

Abstract. Taq Polymerase Is Essential In Polymerase Chain

ABSTRACT Taq Polymerase is essential in Polymerase Chain Reaction(PCR) experiments to obtain a PCR amplification of an unknown gene. The unknown gene is then ligated into a vector plasmid, which is placed in a bacterium Escherichia Coli to transform and multiply. Ultimately, identification and characterization of the unknown gene is done using electrophoreses and gel imaging. Cloning techniques such as the one performed have been used for many years to isolate genes from a variety of species. Isolation of unknown genes serves many purposes. Nucleic acid sequence of the unknown gene can be derived which can be compared to other known gene sequences and the function of the gene can be derived. The clone made can be used to study the†¦show more content†¦The active monomer form of Taq polymerase has a molecular mass of 94 kD with a full length of 832 amino acids. (2) Mainly, Taq polymerase catalyzes the incorporation of dNTPs into DNA and contains a polymerization dependent 5à ¢â‚¬â„¢-3’ DNA polymerase activity. (1) Although there are many advantages towards the use of Taq polymerase in the amplification reaction, Taq polymerase does not possess 3’ to 5’ exonuclease (proofreading) activity. Taq polymerase is purified and tested using SDS-PAGE gel electrophoresis to separate and identify the purity of the sample. The recombinant protein is purified from an E. coli strain containing the pTTQ18 plasmid that contains the Taq DNA polymerase I gene that is cloned downstream Ptac promoter. After purification through a â€Å"salting out† method containing (NH4)2SO4, a small sample of Taq polymerase will be used to be identified through electrophoresis. After enrichment of the protein, the sample of Taq polymerase will be saved to be used in further experiments involving PCR amplification, cloning, and DNA sequencing. Polymerase Chain Reaction is a method developed in the 1980s by Kary Mullis. The main objective to PCR is the ability of the DNA polymerase, in our case Taq polymerase, to synthesize a new strand of DNA that is complementary to the unknown gene offered in the templateShow MoreRelatedSex Determination By Amplification Of Amelogenin Gene From Dental Pulp Tissue By Pcr1727 Words   |  7 PagesSex determination by amplification of Amelogenin gene from dental pulp tissue by PCR Abstract Introduction: Forensic odontology necessarily involves the application of dentistry along with various other branches of sciences to resolve criminal cases with legal issues. Sex determination is a part of forensic odontology and an en essential priority when traditional identification of the deceased becomes impossible due to certain mishaps, including natural calamities, acts of war and terrorism or criminalRead MoreCloning of Enhancer of Zeste Homolog 2 in Forward Orientation Into Escherichia Coli Using Histidine-Tagged Pbluescript Ii Ks+.4372 Words   |  18 PagesTitle: Cloning of Enhancer of Zeste Homolog 2 in forward orientation into Escherichia Coli using histidine-tagged pbluescript II KS+. Abstract Enhancer of Zeste Homolog 2 locus is intensely over expressed in breast and prostate cancer and it’s been established that its promoter inhibition by p53 has led to reduced cell proliferation and invasion (Bracken, 2003; Xiao, 2011). Objective is to clone a forward orientated EZH2 insert into a his-tagged pbluescript. Cloning EZH2 into a histidine-taggedRead MoreThe Erk Mapk Pathway Causes Osteoporosis And Formation During Bone Remodeling1906 Words   |  8 PagesDisregulation of the ERK MAPK pathway causes osteoporosis and osteochondroma in mice Abstract Introduction Bones are dynamic tissues that undergo a constant cycle of fracturing, resorbing, and remodeling. Bone mass is reflected by the coupled balance of osteoblasts, which produce bone matrix proteins and osteoclasts, which degrade bone (Miyamoto, 2003). This opposite and complementary activity between these two lineages of cells maintains the homeostasis of bone resorption and formation duringRead MoreComparative Value Of Real Time And Conventional Pcr1911 Words   |  8 PagesMedical Biochemistry, Faculty of Medicine, Benha University, Benha – Egypt. 2 Department of Gynecology and Obstetrics, Faculty of Medicine, Benha University, Benha – Egypt. *Corresponding author naglaa1270@yahoo.com, naglaa.azab@fmed.bu.edu.eg ABSTRACT Invasive procedures including chorionic villus sampling and amniocentesis in sex-linked diseases increase the risk of fetal loss. Therefore, Noninvasive fetal gender determination using cell-free fetal DNA (cffDNA) in maternal plasma may be promisingRead MoreThe Effect Of Sv40 T Antigen On Two Multiple Fission Microalgae Essay4383 Words   |  18 Pages Myongji University, Gyeonggi 449-728, Republic of Korea *Corresponding author Gyuhwa Chung E-mail: chung@chonnam.ac.kr Phone: +82-61-659-7302 Fax: +82-61-659-7309 Running title: Effects of SV40 T antigen on two multiple fission microalgae â€Æ' ABSTRACT Combination of Simian Virus40 (SV40) large T antigen and its replication origin is commonly used to enhance the expression efficiency of heterogeneous genes in the host cell by increasing the copy number. Most studies on SV40 large T antigen are